Next Generation Sequencing
Yeast Two-Hybrid Screening Services
We offer a suite of services comprising protein-protein interaction (PPI) screens, protein engineering and functional assays. Our clients are in pharma, biotech and academic research.
We use Next-Generation Sequencing (NGS) as the readout, resulting in more complete datasets. Our thoroughly redesigned Y2H system components provide more quantitative and precise measurements of binding partner interactions than conventional Y2H assays. Our platform technology enables us to decipher the full coverage of protein-protein interactions (PPIs). These discoveries have been applied to problems in human health, energy, food security and sustainability.
Basic NGS-Yeast Two-Hybrid Screening
NGS coupled Yeast Two-Hybrid Screening service for finding new protein-protein interactions, for those who want an advanced and comprehensive readout for their screens. With NGS, the screening is more reliable, more precise than the conventional Y2H screening using first-generation capillary sequencing. Expect up to 10x more hits.
For example, you are interested in a protein or a protein domain and you need to get an idea of what its function is. We will clone the gene of interest into a bait vector and screen this gene with a cDNA library from a relevant source (cells, tissue). You will get then a list of genes that encode potential interactors for your bait proteins. Pathway enrichment analysis highlights the biological roles of the bait protein.
Receive more information about next generation yeast two-hybrid screening services.
Skeptical of NGS-yeast two-hybrid screening? Interested, but need more information? Ready to buy, but need a quote?
Contact us to schedule a consultation and we will answer all your questions.