Services
We provide a suite of unique services for our clients in academia and industry.
Next Interactions provides complete, sensitive, and precise measurements of protein-protein interactions. Our Next Generation Interaction Screening (NGIS) technology combines yeast two hybrid (Y2H) with next generation sequencing (NGS). NGIS integrates the latest innovations in sequencing, yeast-based protein interaction mapping techniques, and quantitative cell-based reporters. A quantitative readout provides information-rich data sets with visualizations. We have successfully completed ~300 NGIS screens for our clients in different research disciplines.
NGIS-Yeast Two-Hybrid Screening
Are you interested in a protein or a protein domain and you need to get an idea of what its function is? We will clone the gene of interest into a bait vector and screen this gene with a cDNA library from the proper source. We will provide you with a list of genes that encode potential interactors for your bait proteins. Quantitative statistics for scoring the interactions eliminates false positives that are a major drawback in conventional Y2H.
References:
Review from 2015: https://www.frontiersin.org/articles/10.3389/fgene.2015.00346/full
Recent NGIS article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046355/pdf/pcbi.1008890.pdf
Florian Kraft Dr. rer. nat.
Uniklinik RWTH Aachen
Aachen, Germany
Identify the function of uncharacterized open reading frames
“We did a NGS-based Y2H with the uncharacterized C2orf69 gene and identified four important mitochondrial proteins. Indeed, C2orf69 mutations disrupt mitochondrial function and are a cause for a complex multisystem disease. Next Interactions’ screening work provided crucial support for these conclusions.”
REFERENCES
C2orf69 mutations disrupt mitochondrial function and cause a multisystem human disorder with recurring autoinflammation
https://www.jci.org/articles/view/143078/pdf
NGIS Interactome Discovery
Do you have a very large project with up to hundred or more bait proteins? For example, you want to get an overview of a disease pathway. Or you want to study the infection mechanism of a pathogen that launches multiple effector proteins? Next Interactions has the unique ability to do NGIS screens in high throughput manner (HTP-NGIS), greatly diminishing the cost per screen. We find pathway enrichment and generate interaction networks to elucidate disease mechanisms.
Virginia Walbot, Ph.D.
Stanford University, CA
Exploring transcription factor regulated pathways
“We worked with Next Interactions to conduct a global Y2H screening by NGS to study bHLH-regulated pathways programs in maize anther development. We were able to discover interactions between several critical pathway components and also uncovered an interaction with an unexpected partner. We were exceptionally pleased with this collaboration and we fully endorse Next Interactions’ screening technology.”
REFERENCES
A cascade of bHLH-regulated pathways programs maize anther development.
https://academic.oup.com/plcell/article/34/4/1207/6501455?login=false
Press Release
A collaboration with BASF’s Agricultural Solutions division: HTP-NGIS was applied to uncover targets of Asian Soybean Rust effector proteins in its host plant.
Next Interactions and BASF Agricultural Solutions developed new technology to analyze Asian soybean rust 
Precision Mapping of Interaction Domains
Do you need to map a binding domain on a protein? Next Interactions, in a collaboration with Frank McCormick (UCSF) and Pau Castel (NYU), developed a combinatorial technology to map minimal functional binding domains with Domain Mapping using Yeast Two-Hybrid Next Generation Sequencing (DoMY-Seq). Here, the NGIS technology is applied to characterize interaction domains down to an unprecedented resolution at the single amino acid level.
Curious about DoMY-Seq?
DoMY-Seq: A yeast two-hybrid–based technique for precision mapping of protein–protein interaction motifs
https://www.jbc.org/action/showPdf?pii=S0021-9258%2820%2900009-5
RAS interaction with Sin1 is dispensable for mTORC2 assembly and activity
https://www.pnas.org/doi/epdf/10.1073/pnas.2103261118
DOWNLOAD OUR WHITEPAPER
Delineating the Minimal Functional Binding Domain – How to Probe Protein Structures with DoMY-Seq
Compound / Drug Screening with NGIS Yeast Two-Hybrid
Would you like to find the best out of 1-10 compounds that would disable the interaction of several known proteins with a target in a cancer pathway? Taking advantage of the improved screening format, we are able to directly assess drug effects on protein interactions, either looking for disruption of already known target interactions, or for drug-induced complex assemblies in the presence or absence of a large number of compounds.
Protein Interactions in Synthetic Biology
You have a specific interest in conducting thorough quantitative analyses to explore the impact of protein variants. This application is particularly relevant in the field of synthetic biology, where it can be used, for example, to assess the interactions between synthetic receptors and natural ligands.
References:
Chatterjee P, iScience. 2020 Oct 28;23(12):101670. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756140/pdf/main.pdf
Zhang S., Proc Natl Acad Sci U S A. 2018 Sep 11;115(37) https://www.pnas.org/doi/epdf/10.1073/pnas.1811031115
Shuguang Zhang, Ph.D.
Massachusetts Institute of Technology
Cambridge, MA, USA
Testing Interactions of Synthetic Receptors
“We engineer synthetic proteins with novel properties. Next Interactions is our partner for screening libraries and for the discovery of key variants. This is an excellent team with such dedication, knowledge and enthusiasm.”
QTY code enables design of detergent-free chemokine receptors that retain ligand-binding activities
https://www.pnas.org/doi/epdf/10.1073/pnas.1811031115
Additional Services
One-on-one Interaction Test
Two proteins are present in a pathway and you need a simple confirmation of the interaction. Or you want to see the differences between wild-type and variant of a protein.
Nicolina Sostaric et al, Molecular & Cellular Proteomics, 2018
Conventional Yeast Two-Hybrid Screening
Conventional Yeast Two-Hybrid Service is offered for clients who need simple solutions for their project, and are on a restricted budget. This service includes the sequencing of 96 individual prey clones.
Custom cDNA Libraries
Next Interactions can provide cDNA library construction for your own use in Y2H or in other assays. We can construct cDNA libraries from different tissues either for full-length genes or tailored gene fragments.
Bioinformatics Support and Other Services
We can support your data analysis and visualization of interaction networks and protein structures. Or perhaps you are interested in other screening assays such as protein fragment complementation?
One-on-one Interaction Test
Two proteins are present in a pathway and you need a simple confirmation of the interaction. Or you want to see the differences between wild-type and variant of a protein.
Nicolina Sostaric et al, Molecular & Cellular Proteomics, 2018
Conventional Yeast Two-Hybrid Screening
Conventional Yeast Two-Hybrid Service is offered for clients who need simple solutions for their project, and are on a restricted budget. This service includes the sequencing of 96 individual prey clones.
Custom cDNA Libraries
Next Interactions can provide cDNA library construction for your own use in Y2H or in other assays. We can construct cDNA libraries from different tissues either for full-length genes or tailored gene fragments.
Bioinformatics Support and Other Services
We can support your data analysis and visualization of interaction networks and protein structures. Or perhaps you are interested in other screening assays such as protein fragment complementation?