We don’t make our customer’s discoveries, but many are made with our help.
Hear from a few scientists who have used our services to discover crucial protein-protein interactions for their research.
Gregor Andelfinger, MD, FRCPC
Laboratory of Cardiovascular Genetics, Sainte Justine UHC Research Center, Université de Montréal, Montréal, QC, Canada
Protein Interactions Reveal Functions of Novel Isoforms in Disease
”While working with a cell regulator involved in heart disease we discovered an alternative splicing variant with an intriguing new domain. With Next Interactions, we mapped candidate interactors for this domain, allowing us to reach pivotal conclusions for our project. Great customer care and turnaround!”
George Chuck, Ph.D.
Department of Plant & Microbial Biology, University of California, Berkeley, Berkeley, CA, USA
Systematic exploration of Biological Pathways
“My lab focuses on the transcriptional regulation of the Tillering pathway in Zea mays. Next Interactions did a great job constructing the cDNA library, optimizing our baits, and providing us with high quality data, We obtained a trove of valuable interactions, including a core set of 30 highly connected proteins; these are enough leads to keep us busy, and more than we expected from our previous experiences. Indeed, Next Interaction’s Y2H combined with deep sequencing proves to be the superior approach.”
Nicolas Grillet, Ph.D.
Assistant Professor, Stanford University, Stanford, CA, USA
Differential Protein-Protein Interaction Profiles for Inherited Diseases
”We suspected that mutations in a gene implicated in cochlear function affected its protein-protein interaction profile. We had no possibility of using mass spectrometry for this special type of cells. Instead we used NGS-Y2H to screen a specific library with mutant and wild-type variants. We found key differences that were crucial to our current research.”
Shuguang Zhang, Ph.D.
Massachusetts Institute of Technology, Cambridge, MA, USA
Testing Interactions by Synthetic Proteins
“We engineer synthetic proteins with novel properties. Next Interactions is our partner for screening libraries and for the discovery of key variants. This is an excellent team with such dedication, knowledge and enthusiasm.”
Hao Hu & Fengqun Yu
Saskatoon Research Centre, Agriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada
Large Scale Screening for Interactome Mapping
“We are working on pathogenesis and disease resistance mechanisms in Brassica crops. With Next Interactions’ screening technology we can get a complete overview how pathogen effector proteins interact with plant defense mechanisms, and how plants can eventually achieve resistance. This is the best technique with such capability we can find on the market.”
Jian-Hua Mao, Ph.D.
Lawrence Berkeley National Laboratory, Berkeley, CA, USA
Identification of Targets for Tumor Suppression and Modulation of Cancer Progression
“We study the tumor suppressor FBXW7, a ubiquitin-ligase that targets proteins for degradation and is regulated by other proteins. NGS-based interaction screens reliably identified key targets and regulators, which were confirmed in stringent co-immunoprecipitation assays.”