The next generation of protein interaction assays

“Next Interaction’s NGIS screening with mutant and wild-type version of a gene implicated in hearing disorders revealed distinct interactions that may underlie observed phenotypes. These initial findings were crucial to our current research.”
Nicolas Grillet, Ph.D., Assistant Professor, Stanford University, USA
“With Next Interactions we were able to map candidate interactors to a novel protein epitope, allowing us to reach pivotal conclusions for our project. Great customer care and turnaround!”
Gregor Andelfinger, MD, FRCPC, Université of Montréal, Canada
“Next Interactions is the ideal partner in molecular engineering and screening of synthetic proteins.  This is an excellent team with such dedication, knowledge and enthusiasm.“
Shuguang Zhang, Ph.D., Massachusetts Institute of Technology, USA

Next Generation Screening and Precision Mapping (NGIS) Technology & Services for Protein-Protein Interactions

Women carrying out NGS Y2H screening of Protein-Protein Interactions

Next Interactions, Inc. is a proteomics technology and services biotech company that delivers more complete, sensitive, and precise measurements of protein-protein interactions (PPIs) to our clients in the biopharmaceutical and agricultural fields. We developed next generation sequencing (NGS) coupled yeast two-hybrid (Y2H) for precise mapping of PPI binding sites and for comprehensive screening to find novel protein-protein interactions. As a result, we accelerate drug discovery besides basic research. 

Current services

Our Next Generation Interaction Screening (NGIS) and Precision Mapping platform technology combines yeast two hybrid (Y2H) with next generation sequencing (NGS), bringing conventional Y2H assay to a new level. Therefore it perfectly complements mass-spectrometry based proteomics technologies. Our NGIS platform integrates the latest innovations not only in sequencing, yeast-based protein interaction mapping techniques, but also in quantitative cell-based reporters. Moreover, using cloud-based data delivery, our quantitative screens give scientists information-rich data sets. In addition we offer visualizations and bioinformatics analyses that are clear and actionable.

Our mission

Our mission is to bring the most precise, reliable and comprehensive tool to answer protein interactions questions, whether precision mapping of known functional interactions, screening for novel protein interactions for basic research, in search of a new drug target, or high throughput compound screening for disrupting known interactions. Most importantly, using our assays, our clients would need to do less validation of the protein hits downstream. Therefore, we accelerate the R&D process, save time & money in the long run.

Technology development

Extending our platform, we develop novel technologies to measure protein interactions in the endogenous milieu of mammalian cells. Such novel technologies will be more efficient addressing the effects of compounds on protein interactions than current approaches and could be leveraged in personalised medicine and in difficult to drug diseases such as cancer and orphan diseases. We are looking for strategic partnerships in this area. 

What are a protein-protein interactions or PPIs?

Protein–protein interactions (PPIs) are the specific physical contacts established between two or more protein molecules. Protein-protein interactions fundamentally underlie the function of all biological systems.

Why does measuring protein-protein interactions matter?

Measuring protein-protein interactions is central to understanding the biological basis of disease, because it enables biotechnology to produce products ranging from safer and more effective therapeutics to next-generation foods, materials, and fuels.

Protein Interaction Screening Challenges

Understanding protein interactions can be powerful, but conventional protein interaction screens needed to be improved. Why? 

Puzzle with missing piece illustrating concept of incomplete coverage in protein-protein interactions.

Interaction Coverage

Complete coverage of the interaction space requires analyzing thousands of interaction pairs.

Icon showing Gaussian distribution to illustrate the challenge of quantitation when measuring protein-protein interactions.


Quantitative measurements are needed to assess the significance of the results.

Image of target highlighting the importance of measurement precision.


Conventional Y2H results in too many false positives. Repetition of screens and background controls for statistical rigor reduces false positives.

Data Visualization illustration

Data Visualization

Effective visualization is required to analyze quantitative and comprehensive results.

Consult our Protein Interaction Screening Experts.

Contact Next Interactions for advice from our scientists about the best approach for shedding light on the protein interactions you need to understand for your research. We can advise you on methods such as the NGS-yeast two-hybrid system, yeast two-hybrid system, how to complement interactome coverage by mass spectrometry, and more.

We Take the Yeast Two-Hybrid System to the Next Generation Level So You Don’t Miss an Interaction

Image of DNA

Streamlined Experimental
Screening Tools

Quantitative scoring and comparison to controls reduce false positives.

Illustration of accessing data on the cloud

Cloud-Based Data
Visualization and Display

Access your data anywhere, any time, using our secure data sharing portal.

Illustration of protein gel separation

Advanced Bioinformatics
Tools for Data Analysis

Bioinformatics data analysis provides quantitative statistics.


Image of person viewing multi well plate on microscope

Understanding of Inheritable Disease

With NGS–Yeast Two-Hybrid assays, find variants of unknown significance using parallel interaction fingerprints.

Image showing how DNA is the source of new therapeutics - DNA in a drug capsule

Novel Therapeutic Target Discovery

Generate interaction profiles for novel isoforms and protein variants.

Three Dimensional space-filled model of a protein

Interactome-Driven Bioengineering

Support a protein interaction-driven rationale for protein engineering and synthetic biology.

Image of bacteria colonies plated out and growing

Biotherapeutics Development

Explore the molecular mechanisms in pathogenesis.

Our Partners

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