The Next Generation of Protein Interaction Assays

Next-generation interaction screening (NGIS) is a reinvention of the yeast two-hybrid (Y2H) system that enables a comprehensive and precise identification of individual protein interactions and entire interaction networks.

working with plates at microscope

Basic Research

Discovery of new PPIs,  predict protein function, and compare interaction profiles for multiple proteins and protein isoforms.

Corn in field


Explore large PPI networks and pathways in different species, enabling thorough analysis of host-pathogen interactions.

Scientific equipment

Drug Discovery

Facilitate the discovery of novel drug targets and define mechanisms relevant for disease.

“Next Interaction’s NGIS screening with mutant and wild-type version of a gene implicated in hearing disorders revealed distinct interactions that may underlie observed phenotypes. These initial findings were crucial to our current research.”

Picture of satisfied customer - GrilletNicolas Grillet, Ph.D.
Assistant Professor
Stanford University, USA

Our Services

DNA sequencing


Find novel protein-protein interactions with  a comprehensive screening method: NGIS Yeast Two-Hybrid.

Network diagram


Chart entire interactome networks and pathways with high-throughput next-gen interaction screening: HTP-NGIS.



Map protein binding domains at amino-acid resolution: Protein Domain Mapping using Yeast 2 Hybrid-Next Generation Sequencing: DoMySeq.

Petri dish


Test protein interactions in one-on-one confirmation assays or perform conventional Y2H.

Our offer also include the synthesis of customized cDNA libraries for your in-house Y2H screens and support for downstream data analysis.

Press Release

A collaboration with BASF’s Agricultural Solutions division: HTP-NGIS was applied to uncover targets of Asian Soybean Rust effector proteins in its host plant.

Press Release preview

Consult our Protein Interaction Screening Experts

Contact Next Interactions for advice about the best approach you need to propel your research. We can advise you on the NGIS two-hybrid system, data analysis coverage, and more.

Image of DNA double helix to illustrate screening tools

Streamlined Screening Tools

Quantitative scoring and comparison to controls reduce false positives.

next interactions data visualization

Advanced Bioinformatics

Quantitative scoring to reduce false positives.

next interactions cloud data


Cloud-Based Data
Visualization and Display

Our Partners

Partner logo - Science Exchange
Partner logo - Quintarabio
Partner BIOINFORX logo
Partner - Seqmatic logo