The Next Generation of Protein Interaction Assays
Next-generation interaction screening (NGIS) is a reinvention of the yeast two-hybrid (Y2H) system that enables a comprehensive and precise identification of individual protein interactions and entire interaction networks.
Discovery of new PPIs, predict protein function, and compare interaction profiles for multiple proteins and protein isoforms.
Explore large PPI networks and pathways in different species, enabling thorough analysis of host-pathogen interactions.
Facilitate the discovery of novel drug targets and define mechanisms relevant for disease.
“Next Interaction’s NGIS screening with mutant and wild-type version of a gene implicated in hearing disorders revealed distinct interactions that may underlie observed phenotypes. These initial findings were crucial to our current research.”
Find novel protein-protein interactions with a comprehensive screening method: NGIS Yeast Two-Hybrid.
Chart entire interactome networks and pathways with high-throughput next-gen interaction screening: HTP-NGIS.
Map protein binding domains at amino-acid resolution: Protein Domain Mapping using Yeast 2 Hybrid-Next Generation Sequencing: DoMySeq.
Test protein interactions in one-on-one confirmation assays or perform conventional Y2H.
Our offer also include the synthesis of customized cDNA libraries for your in-house Y2H screens and support for downstream data analysis.
A collaboration with BASF’s Agricultural Solutions division: HTP-NGIS was applied to uncover targets of Asian Soybean Rust effector proteins in its host plant.
Streamlined Screening Tools
Quantitative scoring and comparison to controls reduce false positives.
Quantitative scoring to reduce false positives.
Visualization and Display