The next generation of protein interaction assays
“Next Interaction’s NGIS screening with mutant and wild-type version of a gene implicated in hearing disorders revealed distinct interactions that may underlie observed phenotypes. These initial findings were crucial to our current research.”
“With Next Interactions we were able to map candidate interactors to a novel protein epitope, allowing us to reach pivotal conclusions for our project. Great customer care and turnaround!”
“Next Interactions is the ideal partner in molecular engineering and screening of synthetic proteins. This is an excellent team with such dedication, knowledge and enthusiasm.“
Next Generation Interaction Screening (NGIS) Services for Protein-Protein Interactions
Protein-protein interactions fundamentally underlie the function of biological systems. Measuring protein-protein interactions is central to understanding the biological basis of disease and for enabling biotechnology to produce products ranging from safer and more effective therapeutics to next-generation foods, materials, and fuels.
Next Interactions, Inc. is a proteomics services company that delivers more complete, sensitive, and precise measurements of protein-protein interactions. We have developed a Next Generation Sequencing-Yeast Two Hybrid (NGS-Y2H) technology platform that combines the latest innovations in sequencing, yeast-based protein interaction mapping techniques, and quantitative cell-based reporters.
Using cloud-based data delivery, our quantitative protein-protein interaction screens give scientists information-rich data sets with visualizations and bioinformatics analyses that are clear and actionable.
We offer a variety of protein interactome discovery solutions, including parallel interaction fingerprints, comparative interaction profiles, and complete screening of protein variants and mutational profiles of proteins, in addition to the construction and use of custom cDNA libraries for our clients.
Protein Interaction Screening Challenges
Understanding protein interactions can be powerful, but protein interaction screens need to be improved.
Complete coverage of the interaction space requires analyzing thousands of interaction pairs.
Quantitative measurements are needed to assess the significance of the results.
Repetition of screens and background controls for statistical rigor reduces false positives.
Effective visualization is required to analyze quantitative and comprehensive results.
Consult our Protein Interaction Screening Experts.
Contact Next Interactions for advice from our scientists about the best approach for elucidating the protein interactions you need to understand for your research. We can advise you on methods such as the NGS-yeast two-hybrid system, yeast two-hybrid system, how to compliment interactome coverage by mass spectrometry, and more.
We Take the Yeast Two-Hybrid System to the NextGen So You Don’t Miss an Interaction
Quantitative scoring and comparison to controls reduce false positives.
Visualization and Display
Access your data anywhere, any time, using our secure data sharing portal.
Tools for Data Analysis
Bioinformatics data analysis provides quantitative statistics.
Understanding of Inheritable Disease
With NGS–Yeast Two-Hybrid assays, find variants of unknown significance using parallel interaction fingerprints.
Novel Therapeutic Target Discovery
Generate interaction profiles for novel isoforms and protein variants.
Support a protein interaction-driven rationale for protein engineering and synthetic biology.
Explore the molecular mechanisms in pathogenesis.