The next generation of protein interaction assays

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“Next Interaction’s NGIS screening with mutant and wild-type version of a gene implicated in hearing disorders revealed distinct interactions that may underlie observed phenotypes. These initial findings were crucial to our current research.”
Nicolas Grillet, Ph.D., Assistant Professor, Stanford University, USA

“With Next Interactions we were able to map candidate interactors to a novel protein epitope, allowing us to reach pivotal conclusions for our project. Great customer care and turnaround!”
Gregor Andelfinger, MD, FRCPC, Université of Montréal, Canada

“Next Interactions is the ideal partner in molecular engineering and screening of synthetic proteins. This is an excellent team with such dedication, knowledge and enthusiasm.“
Shuguang Zhang, Ph.D., Massachusetts Institute of Technology, USA

Next Generation Interaction Screening and Mapping (NGIS) Services for Protein-Protein Interactions

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Protein-protein interactions fundamentally underlie the function of biological systems.

What is a protein-protein interaction or PPI?

Protein–protein interactions are the physical contacts of high specificity established between two or more protein molecules.

Why measuring protein-protein interactions matters? Measuring protein-protein interactions is central to understanding the biological basis of disease and for enabling biotechnology to produce products ranging from safer and more effective therapeutics to next-generation foods, materials, and fuels.

Next Interactions, Inc. is a proteomics services company that delivers more complete, sensitive, and precise measurements of protein-protein interactions. Our Next Generation Interaction Screening and Mapping platform technology (NGIS) combines yeast two hybrid with next generation sequencing (NGS-Y2H), bringing conventional yeast two hybrid to the level of mass spectrometry approach. Our NGIS platform integrates the latest innovations in sequencing, yeast-based protein interaction mapping techniques, and quantitative cell-based reporters. Using cloud-based data delivery, our quantitative protein-protein interaction screens give scientists information-rich data sets with visualizations and bioinformatics analyses that are clear and actionable.

Our goal is to bring the most reliable and comprehensive tool to answer your protein interactions questions, whether screening for novel protein interactions for basic research or in search of a new drug target, or precision mapping of known functional interactions, or compound screening. Ultimately, we accelerate the R&D process for you, save you time & money and get you the results you need.

Protein Interaction Screening Challenges

Understanding protein interactions can be powerful, but conventional protein interaction screens needed to be improved. Why?

Interaction Coverage

Complete coverage of the interaction space requires analyzing thousands of interaction pairs.

Quantitation

Quantitative measurements are needed to assess the significance of the results.

Precision

Conventional Y2H results in too many false positives. Repetition of screens and background controls for statistical rigor reduces false positives.

Data Visualization

Effective visualization is required to analyze quantitative and comprehensive results.

Consult our Protein Interaction Screening Experts.

Contact Next Interactions for advice from our scientists about the best approach for elucidating the protein interactions you need to understand for your research. We can advise you on methods such as the NGS-yeast two-hybrid system, yeast two-hybrid system, how to compliment interactome coverage by mass spectrometry, and more.

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We Take the Yeast Two-Hybrid System to the NextGen So You Don’t Miss an Interaction

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Streamlined Experimental Screening Tools

Quantitative scoring and comparison to controls reduce false positives.

Cloud-Based Data
Visualization and Display

Access your data anywhere, any time, using our secure data sharing portal.

Advanced Bioinformatics
Tools for Data Analysis

Bioinformatics data analysis provides quantitative statistics.

Applications

Understanding of Inheritable Disease

With NGS–Yeast Two-Hybrid assays, find variants of unknown significance using parallel interaction fingerprints.

Novel Therapeutic Target Discovery

Generate interaction profiles for novel isoforms and protein variants.

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Interactome-Driven Bioengineering

Support a protein interaction-driven rationale for protein engineering and synthetic biology.

Biotherapeutics Development

Explore the molecular mechanisms in pathogenesis.