The next generation of protein interaction assays

“Next Interaction’s NGIS screening with mutant and wild-type version of a gene implicated in hearing disorders revealed distinct interactions that may underlie observed phenotypes. These initial findings were crucial to our current research.”
Nicolas Grillet, Ph.D., Assistant Professor, Stanford University, USA

“With Next Interactions we were able to map candidate interactors to a novel protein epitope, allowing us to reach pivotal conclusions for our project. Great customer care and turnaround!”
Gregor Andelfinger, MD, FRCPC, Université of Montréal, Canada

“Next Interactions is the ideal partner in molecular engineering and screening of synthetic proteins.  This is an excellent team with such dedication, knowledge and enthusiasm.“
Shuguang Zhang, Ph.D., Massachusetts Institute of Technology, USA

Next Generation Interaction Screening (NGIS) Services for Protein-Protein Interactions

Women pipetting samples and reagents in the lab

Protein-protein interactions fundamentally underlie the function of biological systems.  Measuring protein-protein interactions is central to understanding the biological basis of disease and for enabling biotechnology to produce products ranging from safer and more effective therapeutics to next-generation foods, materials, and fuels.

Next Interactions, Inc. is a proteomics services company that delivers more complete, sensitive, and precise measurements of protein-protein interactions.  We have developed a Next Generation Sequencing-Yeast Two Hybrid (NGS-Y2H) technology platform that combines the latest innovations in sequencing, yeast-based protein interaction mapping techniques, and quantitative cell-based reporters.

Using cloud-based data delivery, our quantitative protein-protein interaction screens give scientists information-rich data sets with visualizations and bioinformatics analyses that are clear and actionable.

We offer a variety of protein interactome discovery solutions, including parallel interaction fingerprints, comparative interaction profiles, and complete screening of protein variants and mutational profiles of proteins, in addition to the construction and use of custom cDNA libraries for our clients.


Consult our Protein Interaction Screening Experts.

Contact Next Interactions for advice from our scientists about the best approach for elucidating the protein interactions you need to understand for your research. We can advise you on methods such as the NGS-yeast two-hybrid system, yeast two-hybrid system, how to compliment interactome coverage by mass spectrometry, and more.

Request a free consultation

Protein Interaction Screening Challenges

Understanding protein interactions can be powerful, but protein interaction screen need to be improved.

Puzzle with missing piece illustrating concept of incomplete coverage in protein-protein interactions.

Interaction Coverage

Complete coverage of the interaction space requires analyzing thousands of interaction pairs.

Icon showing Gaussian distribution to illustrate the challenge of quantitation when measuring protein-protein interactions.


Quantitative measurements are needed to assess the significance of the results.

Image of target highlighting the importance of measurement precision.


Repetition of screens and background controls for statistical rigor reduces false positives.

Data Visualization illustration

Data Visualization

Effective visualization is required to analyze quantitative and comprehensive results.

We Take the Yeast Two-Hybrid System to the NextGen So You Don’t Miss an Interaction

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Streamlined Experimental
Screening Tools

Quantitative scoring and comparison to controls reduce false positives.

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Cloud-Based Data
Visualization and Display

Access your data anywhere, any time, using our secure data sharing portal.

Illustration of protein gel separation

Advanced Bioinformatics
Tools for Data Analysis

Bioinformatics data analysis provides quantitative statistics.


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Understand Inheritable Disease

With NGS–Yeast Two-Hybrid assays, find variants of unknown significance using parallel interaction fingerprints.

Image showing how DNA is the source of new therapeutics - DNA in a drug capsule

Discover Novel Therapeutic Targets

Generate interaction profiles for novel isoforms and protein variants.

Interactome-Driven Bioengineering

Support a protein interaction-driven rationale for protein engineering and synthetic biology.

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Biotherapeutics Development

Explore the molecular mechanisms in pathogenesis.

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